Figure 2
From: Functional transformations of bile acid transporters induced by high-affinity macromolecules
Physical interactions of ASBT and LHe-tetraD in cells.
(a) Immunoprecipitation-immunoblot analysis for p-ASBT (IP-ASBT, IB-pTyr) and the total ASBT (IP-ASBT, IB-ASBT) from the non-treated and LHe-tetraD-treated MDCK-ASBT cells. (b) Biotin-labeled LHe-tetraD was incubated with MDCK-ASBT cells and pulled-down by streptavidin beads from membrane and cytoplasmic fraction as well as from the whole cell lysates, followed by immunoblotting for bound ASBT protein and biotin. The control included the ASBT band from lysates and the immunoprecipitates of the non-treated MDCK-ASBT cells. Asterisk indicates non-specific band; (c) The co-localization of LHe-tetraD (red) and ASBT (green) were also visualized in MDCK-ASBT cells. Scale bar indicates 20 μm.
