Figure 3
From: Functional transformations of bile acid transporters induced by high-affinity macromolecules
Visualization of ASBT and LHe-tetraD complex internalization in cells.
(a) Proxitmity ligation assay (PLA; red dot) between ASBT and biotin-labeled LHe-tetraD in MDCK-ASBT treated cells. Scale bar indicates 20 μm. (b) The co-localization of PLA-labeled ASBT/LHe-tetraD complexes (green) with early endosome marker EEA-1 (red) in MDCK-ASBT cells. Scale bar indicates 20 μm. The co-localization analysis performed on original images using Van Steensel's cross-correlation coefficient (CCF) between PLA labeled ASBT/LHe-tetraD and EEA-1 with JACop software. The perfect bell-shaped curves and Pearson coefficient (r) ranging from 0.8 to 1 were observed for the ASBT/LHe-tetraD complexes with EEA-1 (right panel).
