Figure 6
From: Functional transformations of bile acid transporters induced by high-affinity macromolecules
Redistribution of ASBT in vivo after LHe-tetraD treatment.
(a) The internalization of ASBT from the apical membrane (arrow) to the sub-apical mucosa (arrowhead) of rat ileum following treatment with LHe-tetraD. At 24 h after LHe-tetraD administration, ASBT were recycled back to the apical region as that of the control group. (b) Note that the total ileum ASBT protein expression was not changed. IBABP staining and expression in the sub-apical mucosa (arrowhead) of rat ileum following treatment with LHe-tetraD as shown by immunohistochemistry (c) and western blot analysis (d) from the whole-tissue lysates. Scale bars indicate 50 μm. To make a better representation, the blots are cropped and the full-length blots are presented in the supplementary Figure S7.
