Figure 4

SIRT1-expressing adenovirus infection and IFN-β treatment inhibit LPS-induced lethal endotoxic and septic shock.

(A) Survival rate of mice infected with SIRT1-expressing adenovirus in response to LPS challenge or cecal ligation puncture. C57BL/6 mice were randomly grouped and injected with LacZ or SIRT1 expressing adenovirus (1 × 10¹⁰ MOI). Two days later, the mice were injected with 18 mg/kg body weight of LPS and ligation and puncture of the cecum (CLP) or sham operation was performed. SIRT1 expression in the liver and spleen of mice infected with SIRT1 expressing adenovirus was checked by western blot (upper panel). The survival rates at different times after LPS challenge (middle panel) or CLP (lower panel) are shown. (B) Survival rate of mice treated with IFN-β in response to LPS challenge or cecal ligation puncture. C57BL/6 mice were injected with vehicle or IFN-β (1000 U/20 g). SIRT1 expression in the liver and spleen of mice treated with IFN-βwas checked by western blot (upper panel). Thirty min later, the mice were injected with 18 mg/kg body weight of LPS and ligation and puncture of the cecum or sham operation was performed. The survival rates at different times after LPS challenge (middle) or CLP (lower) are shown. Full-length blots are presented in Supplementary Figure S8–S9. The Western blot shown is a representative of three independent experiments.