Figure 1
From: Structure and mutagenesis of the DNA modification-dependent restriction endonuclease AspBHI
AspBHI is a member of MspJI family.
(a) Schematic representation of AspBHI and members of MspJI family. The conserved region is shown in dark grey and insertions are shown in open boxes. (b) Sequence alignment of AspBHI and members of MspJI family. The AspBHI residue numbering is shown above the sequence alignment. The pairwise comparison of AspBHI and MspJI was shown previously7. Amino acids highlighted are either invariant (white against black) among the five proteins or similar (white against grey) as defined by the following groupings: V, L, I and M; F, Y and W; K and R, E and D; Q and N; E and Q; D and N; S and T; and A, G and P. Helices are labeled αA-αM; strands are labeled β1–β15 (strand β8 is subdivided into β81 and β82 owing to a discontinuity in this strand). (c) Distribution of averaged crystallographic thermal B factor per residue.
