Figure 2
Human IAPP oligomers Toxicity.
(a) Rin-m cells treated with hIAPP oligomers (grey) or with rIAPP (black) in diverse concentrations. Cells viability was estimated by MTT reduction assay (*P < 0.05, **P < 0.005). (b) Dye leakage from calcein containing liposomes. 1 μM hIAPP oligomers (green rectangle) or rIAPP (blue rhombus) were incubated with the liposomes and membrane damage was evaluated by increased fluorescence (excitation: 495, emission: 520) and compared to the control group (red square). (c) FACS results of the incubation of Rin-m cells with hIAPP oligomers at different concentrations. The Annexin V-FITC apoptosis detection kit was used for the detection of apoptotic cells. FL1-H is the fluorescence of V-FITC and FL2-H is the fluorescence of Annexin V-PE. I-cells in necrotic state, II- late apoptotic state, III- early apoptotic state and IV- viable cells (d) Diagram presentation of cell state dispersion of three FACS analysis assays, as presented in figure 2C, dark grey column represent viable cells, light grey column represent early and late apoptotic cells and black column represent necrotic cells.
