Figure 1
SlFYFL sequence and expression analysis in AC (WT).
(A) Phylogenetic analysis of FYFL and other MADS-Box proteins was constructed by the neighbor-joining method, bootstrap analysis of 1000 replicates. FYFL is marked with asterisk. Accession numbers for the proteins listed are as follows: SlMADS-MC (NP_001234665), SlTDR4 (FUL1) (NM_001247244), SlMADS-RIN (NM_001247741.1), SlMADS1 (NP_001234380), AtAGL42 (AY141213), SlFYFL(KF709444), JOINTLESS (AAG09811). (B) Multiple sequence alignment of FYFL and other MADS-Box proteins. SlFYFL is marked with asterisk. Identical amino acids are shaded in black and similar amino acids are shaded in gray. (C) The relative expression patterns of FYFL in AC. IMG, immature green fruit; MG, mature green fruit; B, breaker fruit; B + 4, 4 days after breaker fruit; B + 7, 7 days after breaker fruit; Yl, young leaf; Ml, muture leaf; Sl, senescent leaf; R, root; ST, stem; S, sepal of flower in anthesis; AZ, abscission zone of flower in anthesis. The relative expression of FYFL in different stages AZ (D) and different stages sepal (E) of AC. MG, mature green fruit; B, breaker fruit; B + 4, 4 days after breaker fruit; B + 7, 7 days after breaker fruit; B + 14, 14 days after breaker fruit. Ys, young sepal; Ms, mature sepal; Ss, senescent sepal. The data represent mean from three replicates with three biological repeats. Error bars indicate SE.
