Figure 2 | Scientific Reports

Figure 2

From: Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry

Figure 2

Purification of AMPK subunits in control versus target myc-AMPK IP groups.

(A) Lysates from HEK293T cells and INS-1 cells expressing pcDNA3.1 or Myc-AMPK α1/β1 were immunoblotted with anti-c-Myc. (B) Lysate from HEK293T cells expressing pcDNA3.1 or Myc-AMPK α1/β1 was incubated with anti-c-Myc-conjugated Dynabeads and covalently coupled to matrices for incubation with INS-1 cell lysate. Bound proteins were eluted and subjected to western blot with anti-AMPK-α1 and -β1.

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