Figure 3 | Scientific Reports

Figure 3

From: Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry

Figure 3

Strategy of SAINT analysis for interactome.

To remove nonspecific interactors, we used stringent filtering criteria and the SAINT algorithm. (A) Flowchart of data processing for AMPK interactome. MS datasets from the pulldown assay and direct IP were filtered sequentially with the filtering criteria. Further, SAINT, species affiliation and BLAST analysis were performed consecutively to differentiate high-confidence interactors and nonspecific contaminants. (B) Identification of proteins that interact with AMPK-α1 and -β1 subunits. The number of identified proteins is shown for each stage of the data processing. AMPK-α1 pulldown assay, AMPK-β1 pulldown assay, AMPK-α1 direct IP and AMPK-β1 direct IP are labeled in blue, red, green and orange, respectively.

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