Table 1 Summary of mass spectrometry analysis
Identifications | Filtered data set by SAINT [Identity ≥ 0.99] | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Proteins | Unique Peptides | Unique Spectra | Unique proteins | |||||||||||
Exp. | IP Baits | Control baits | Target baits | Total Number of Protein | Control baits | Target baits | Total Number of Peptide | Control baits | Target baits | Total Number of Spectra | Rat Only | Rat & Human Concurrently | Human Only | Total Number of Protein |
Direct IP_Bio1 | AMPK-α1 | 278 | 349 | 352 | 1092 | 2194 | 2382 | 1284 | 2602 | 2877 | 273 | - | - | 273a (271)b |
AMPK-β1 | 201 | 216 | 223 | 1035 | 1143 | 1507 | 1227 | 1322 | 1833 | 163 | - | - | 163 (162) | |
Direct IP_Bio2 | AMPK-α1 | 164 | 218 | 220 | 560 | 1322 | 1400 | 643 | 1527 | 1640 | 175 | - | - | 175 (173) |
AMPK-β1 | 119 | 132 | 135 | 536 | 749 | 876 | 619 | 898 | 1057 | 104 | - | - | 104 (102) | |
Pull down_Bio1 | AMPK-α1 | 283 | 596 | 599 | 844 | 3377 | 3626 | 1050 | 4249 | 4573 | 52 | 64 | 58 | 174 (173) |
AMPK-β1 | 291 | 641 | 645 | 842 | 3944 | 4206 | 1048 | 5026 | 5377 | 60 | 74 | 62 | 196 (193) | |
Pull down_Bio2 | AMPK-α1 | 140 | 284 | 287 | 619 | 2135 | 2302 | 785 | 2846 | 3064 | 47 | 31 | 37 | 115 (112) |
AMPK-β1 | 122 | 246 | 248 | 571 | 1941 | 2078 | 704 | 2417 | 2610 | 36 | 31 | 33 | 100 (99) | |
