Table 2 Catalytic properties of different multifunctional enzyme systems[a]

From: Thin-Layer Polymer Wrapped Enzymes Encapsulated in Hierarchically Mesoporous Silica with High Activity and Enhanced Stability

Sample

Rapprent (U/mg)

KM (mM)

kcat (min−1)

GOD + HRP

165

112 ± 8.9

5.0 ± 0.2

GNC-HNC-HMS

162

119 ± 8.1

4.8 ± 0.3

GOD-HRP-HMS

136

136 ± 9.8

3.3 ± 0.1

GOD + HNC-HMS

145

127 ± 8.5

3.8 ± 0.2

HRP + GNC-HMS

149

131 ± 9.1

3.6 ± 0.1

GNC-HNC-HMS[b]

151

132 ± 9.3

3.7 ± 0.2

  1. [a]Reactions were carried out at 25°C in 100 mM phosphate buffer (pH = 4.5) using 1.0 mL 1.0 mg/mL β-D-glucose solution, 100 μL 1.8 mM ABTS, 0.1 mg GOD enzyme and 0.1 mg HRP enzyme.
  2. [b]Rection in in a 96-well plate (5000 HeLa cells/well), 0.1 mg GNC-HNC-HMS catalyst, 0.1 mL 0.2 mg/mL β-D-glucose solution, 10 μL 0.5 mM ABTS.
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