Figure 4
The tetrameric peptides display discrete binding profiles across a panel NCSCL cell lines as well as other cancer types.
(A). Peptide binding was determined by flow cytometry. Representative histograms are shown for HBEC3, H2009 and H1299 cells. Nonspecific uptake and fluorescence controls include cells incubated without SAPE, unconjugated SAPE and a scrambled sequence control peptide conjugated to SAPE. In each case a corresponding scrambled peptide is shown on the line before the targeting peptide. (B). Binding profiles of the free peptides for different cell lines. Cells were considered to be positively stained if their fluorescence intensity was above the first decade. To compare staining across the panel of cells, a staining score was defined as the percentage of positively stained gated live, single cells multiplied by the mean fluorescence intensity of that population. The average and SEM of three experiments are shown. Staining score averages below 5000 are considered negative for significant uptake of the individual peptide conjugate.
