Figure 5
Peptide binding correlates with cellular phenotypes.
Cells were dichotomized into high and low binders using a cell binding score as 5000 as a benchmark and compared to expression levels of 200 proteins analyzed by RPPA. (A). Ten proteins have differential expression of >2-fold between cell lines which are H2009.1 binders and non-binders (t-test, P value <0.01). (B). Epithelial or mesenchymal phenotype of the cell lines correlates with peptide binding (Chi-square analysis, P = 0.0138). Cluster 1 binds H2009.1, H1299.2, HCC15.1 and HCC15.2 peptides. Cluster 2 binds H1299.2, HCC15.1 and HCC15.2 peptides. Cluster 3 binds none of the 4 peptides. Distribution of E-cadherin and N-cadherin expression level amongst each cluster is shown (Anova analysis).
