Figure 8
From: FALCON: fast and unbiased reconstruction of high-density super-resolution microscopy data
FALCON performance on live ER data.
Live imaging of the endoplasmic reticulum protein, reticulon-4 fused to tdEos imaged over 20 seconds in a U2OS cell. Conventional (a–e) and super-resolution (f–j) snapshots are shown with a 2.5 seconds temporal resolution. The average size of tubules measured from the reconstruction is approximately 60 nm (at FWHM). Yellow markers in (g) highlight a representative tubule width. The dynamic motions of these structures are highlighted in (g–j) indicated by white and blue arrows and dashed, yellow circles. All scale bars are 1 μm.
