Figure 1
From: miR-34c attenuates epithelial-mesenchymal transition and kidney fibrosis with ureteral obstruction
Experimental models of EMT and epithelialization revealed miR-34c presumably involves in EMT.
(A, B) PCR and Western blot showing the expression of genes regarding EMT in kidneys of UUO and sham operated mice. Sham right; right kidneys of sham-operated mice. Sham left; left kidneys of sham-operated mice. UUO contralateral; contralateral kidneys of UUO-operated mice. UUO obstructed; UUO kidneys of UUO-operated mice. The original PCR and whole blot pictures are available in Supplementary Fig. S2 (n = 3). (A) PCR normalized to GAPDH. (B) Western blot. (C) Real-time PCR showing the expression of Snail1, Vimentin and KSP in MCT with or without TGF-β stimulation. (D) Real-time PCR showing the expression of KSP in mouse ES cells differentiated with or without Activin 10 ng/ml. (E) Venn diagram of microarray of three experimental models. (F) Real-time PCR of miRNAs in UUO mice. Control; contralateral kidneys of UUO-operated mice. UUO; UUO kidneys of UUO-operated mice. (G) Real-time PCR of miRNAs in MCT stimulated by TGF-β. (H, I) Real-time PCR of miR-34a and miR-34b in (H) UUO mice and (I) MCT stimulated by TGF-β.
