Figure 2
From: miR-34c attenuates epithelial-mesenchymal transition and kidney fibrosis with ureteral obstruction
miR-34c suppressed Jag1 and attenuated EMT induced by TGF-β in MCT.
(A) The protocol of transfection of miR-34c inhibitor or precursor. (B) Real-time PCR showing the expression of Snail1, KSP and miR-34c in MCT treated by TGF-β for 2 days. (C, D) Real-time PCR showing the expression of Snail1, KSP and miR-34c in MCT under indicated conditions. (C) Inhibitors of miR-34c. (D) Precursors of miR-34c. (E) PCR showing the expression of genes regarding Notch signal in MCT under indicated conditions. (F) Western blot of Jag1 and β-actin in MCT under indicated conditions. (n = 2) The original whole blot picture is available in Supplementary Fig. S3.
