Figure 4
From: Mechanical activation of vinculin binding to talin locks talin in an unfolded conformation
Vinculin Vd1 dissociates from talin at high force.
(a) Time-lapse data of talin rod in 0 nM (red) and 10 nM (black) of Vd1 during force jumping between 6 pN for 1 min, during which no domain refolding was observed (pink/grey) and 40 pN for 5 s (red/black). For clarity, the curves are shifted along the extension-axis to avoid overlapping. Inset shows histogram of the unfolding step sizes at 40 pN. (b) Number of Vd1 dissociation events observed with the same procedure as in (a) as a function of Vd1 concentration. Error bar denotes standard deviation. (c) Maximally five Vd1 dissociation events have been observed in rare cases with strong tethers when stretched from 35 pN to 60 pN at a constant loading rate of 1 pN/s.
