Figure 2

DHM regulates survival signals and activates the apoptosis-related signaling pathway.
(A) The expression of apoptosis-related proteins in HepG2 cells after DHM treatment. (B) The expression of apoptosis-related proteins in HL7702 cells after DHM treatment (12 h DHM treatment). (C) Activated caspase proteins (cleaved caspases) were quantified by optical density analysis. (D) AKT and p-AKT proteins were reduced in HepG2 cells after DHM stimulation (12 h treatment). (E) AKT and p-AKT proteins were downregulated in SMMC-7721 but not affected in HL7702 cells after DHM stimulation. (F, G) p-AKT and AKT proteins were quantified by optical analysis in HepG2, SMMC-7721 and HL7702 cells.