Figure 2 | Scientific Reports

Figure 2

From: High-throughput identification and dendritic cell-based functional validation of MHC class I-restricted Mycobacterium tuberculosis epitopes

Figure 2

Identification of HLA-A*0201-restricted epitopes endogenously processed and presented by Mtb antigen encoding RNA-transfected DCs using T2 cells as targets.

Mtb antigen-specific T cells were generated as described in Methods. Europium-labeled T2 cells were incubated with the peptides (100 μM) for 1 h prior to addition of T cells followed by a standard 4 h europium release assay. Negative control (C) is an HLA-A*0201 peptide with a low cScore. Standard deviation reflects variation between triplicate wells. This experiment was done 3 times and data from each experiment is shown in Supplementary Table 2a–c. (2A). Mtb Apa, (2B). Mtb8.4, (2C). Mtb19.

Back to article page