Table 1 Passaging protocols for hPSC culture
From: Enzyme-free Passage of Human Pluripotent Stem Cells by Controlling Divalent Cations
Passage Protocols | Culture Conditions | References | ||||
|---|---|---|---|---|---|---|
Divalent cations | Enzymes | Manipulation | Cell clump size | Serum replacement | Coat** & feeder | Â |
Ca2+, Mg2+ * | Collagenase | Cutting, Scraping, Glass beads | Large | KSR, | MEF | |
Ca2+, Mg2+ * | Free | Cutting | Large | KSR | MEF | |
Ca2+ *** | Trypsin & Collagenase | Pipetting | Large | KSR | MEF | |
Free (EDTA) | Trypsin TrypLE | Pipetting | Single cells, Large | KSR | MEF | |
Free (EDTA) | Free (CDB#) | Pipetting | Single cells | KSR | MEF | |
Ca2+, Mg2+ * | Dispase, Collagenase TrypLE | Scraping, Pipetting | Large | Free | Mg, Gx, Lm, Vn, Fb, Cg, pVn, pBSP | |
Ca2+, Mg2+ * | Free | Cutting | Large | Free | Vn | |
Free (EDTA) | Free (CDB#) | Pipetting | Small | Free | Mg, HBP | |
Ca2+ | Free | Pipetting | Large (Small) | Free | Fb | Present study |
