Figure 5
From: Mast cell-derived serine proteinase regulates T helper 2 polarization
mMCP-6 modulate the expression of GATA3 and Foxp3 in Th2 cells.
From the OTII mouse spleen, we prepared naïve CD4+ T cells and polarized Th2 cells. The polarized Th2 cells (106 cells/ml) were cultured in the presence of mMCP-6 (50 μM) for 48 h as denoted on the x axis. The cellular extracts were analyzed by qRT-PCR and Western blotting. (A–B), the bars indicate the mRNA levels of GATA3 (A) and Foxp3 (B). (C–D), the immune blots indicate the contents of protein of GATA3 (C) and Foxp3 (D). The bars below the immune blots show the summarized integrated density of the blots. The data in bar graphs were presented as mean ± SD from 3 experiments. Naive: Naïve CD4+ T cells. Medium: Cells were cultured with medium alone. mMCP6: Cells were cultured in the presence of mMCP-6. $: The CD4+ T cells were Bcl-6 deficient. Bcl6-Oex: Polarized OTII Th2 cells were generated using a Bcl-6 expressing plasmid to make the cells over express Bcl-6. *, p < 0.01, compared with naive T cell group.
