Figure 6
From: Mast cell-derived serine proteinase regulates T helper 2 polarization
The generated Tregs have an immune suppressor function.
The generated Tregs, DCs and polarized OVA-specific Th2 cells (labelled with CFSE) were prepared as described in the text and cultured in the presence of OVA in culture for 3 days under the following conditions: (A) Medium, Th2 cells were cultured with medium. (B) No Treg, Th2 cells were cultured in the presence of OVA, but no Treg. (C) With Treg, Th2 cells were cultured in the presence of OVA and Treg. (D) Treg + aTGF-β, Th2 cells were cultured in the presence of OVA, Treg and anti-TGF-β pAb (200 ng/ml). (E) Treg + isoIgG, Th2 cells were cultured in the presence of OVA, Treg and isotype IgG (100 ng/ml, using as a control antibody). (A–E), the histograms indicate the frequency of Th2 cell proliferation. (F) is a CFSE-staining control. (G), the bars indicate the summarized data of (A–E). (H), the bars indicate the levels of IL-4, IL-5 and IL-13 in the culture supernatants. The labels of G and H are the same as that in the flow cytometry histograms. Data of (G) and (H) are presented as mean ± SD. *, p < 0.01, compared with group A. #, p < 0.05, compared with group B. The data represent 3 experiments.
