Figure 1 | Scientific Reports

Figure 1

From: Nitroheterocyclic compounds are more efficacious than CYP51 inhibitors against Trypanosoma cruzi: implications for Chagas disease drug discovery and development

Figure 1

The nitroheterocyclics benznidazole and nifurtimox are the most efficacious compounds against a phylogenetically broad panel of Trypanosoma cruzi.

Tissue cultures in 384-well plates were infected with one of the following T. cruzi: Dm28c (DTU I, purple), Y (DTU II, red), ARMA13 cl1 (DTU III, orange), ERA cl2 (DTU IV, light green), 92-80 cl2 (DTU V, light blue), CL Brener (DTU VI, dark blue) and Tulahuen (DTU VI, dark green). Compounds were 2-fold diluted and were added to infected cultures 24 h after T. cruzi plating and incubated for 72 (Dm28c) or 96 h (all others) prior to assessment of antiparasitic activity by high content analysis. Dose-response curves of compounds antiparasitic activity normalized to infected and non-infected controls are shown. The X-axis shows log of compound molar (M) concentrations and Y-axis shows the normalized activity, based on the measurement of ratio of infected cells. Data refers to mean values of at least two independent experiments.

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