Figure 3
From: Automated single-cell motility analysis on a chip using lensfree microscopy
Cell motility as a function of stiffness and surface protein.
(a) Representative ‘Displacement vs. Time’ plots for each experimental condition (0.4 kPa, 4 kPa, 40 kPa PA hydrogels coated with either fibronectin (FN) or collagen type IV (Col-IV)). Single-cell displacement from the initial position of the cell is shown over the course of 20 hrs. These plots reflect only one of the three experiments done for each set of conditions. (b) ‘Displacement vs. Time’ plots averaged across all three trials for each condition. Cells cultured on FN-coated gels were generally less motile than those cultured on gels coated with Col-IV, except in the 0.4 kPa case (most flexible). Thick lines represent the mean and thin lines represent the standard error of the mean (SEM). (c) Average diffusion coefficients calculated for each experimental condition. Average diffusivity was positively correlated with gel stiffness for both proteins. The error bars represent SEM.
