Figure 4
From: Automated single-cell motility analysis on a chip using lensfree microscopy
Standard measurements of cell motility using 10 minute and 30 minute intervals.
(a) Mean cell velocity and mean track length over 20 hrs. Mean cell velocity in μm/min, shown on the left y-axis, was calculated by dividing the mean displacement per interval by the interval time. Mean track length in mm, shown on the right y-axis, was calculated by averaging the sum of the displacements from each interval. Thus, the two values are related by a constant equal to the total time of tracking, i.e., 20 hrs. (b) ‘Directional Persistence’, defined as net displacement divided by corresponding track length, over the full 20 hrs. The differences between 10 minute interval and 30 minute interval measurements reflect the loss of information associated with lower frequency sampling. The error bars indicate the standard error of the mean (SEM). Specific values for each measurement and SEM are reported in Supplementary Table 1.
