Figure 2
From: Integrating reductive and synthetic approaches in biology using man-made cell-like compartments
Ultrahigh-throughput reconstruction of the β-Galactoside hydrolysis subsystem.
(a) Fluorescent reporter for β-galactoside hydrolysis activity. When LacZ that hydrolyzes β-galactosides is expressed in a liposome, CMFDG is hydrolyzed and fluoresces. (b) FACS analysis of the liposome library. When liposomes containing the PURE system solution, CMFDG, a transferrin-Alexa Fluor 647 conjugate and 5 nM E. coli ORF library were constructed, liposomes that emitted CMFDG-derived fluorescence (upper right quadrant) occurred at a rate of 0.26%. No fluorescence was observed for liposomes without the E. coli ORF library.
