Figure 5 | Scientific Reports

Figure 5

From: A high-performance thioredoxin-based scaffold for peptide immunogen construction: proof-of-concept testing with a human papillomavirus epitope

Figure 5

Immune cross-reactivity profiles of anti-PfTrx, anti-EcTrx and anti-MmTrx antibodies.

Sera from mice immunized with empty PfTrx, EcTrx and MmTrx (left graphs in panels a, b and c, respectively) were analyzed by ELISA using the corresponding GST-fusion proteins plus human thioredoxin (GST-HsTrx) as capture antigens. Data obtained with sera from mice immunized with the corresponding L2(20-38)3 insert-containing antigens are shown in the right graphs (panels a-c, as above). Each dot represents an individual mouse serum; mean absorbance values measured with each of the four capture antigens are indicated by horizontal lines. The immunization protocol was the same as the one utilized for the experiments reported in Fig. 4b (see ‘Methods’ for further details).

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