Figure 2
From: In vivo therapeutic potential of Dicer-hunting siRNAs targeting infectious hepatitis C virus.
Efficacy against heterogeneous viruses by mixtures of dh-siRNAs.
(a) Alignment of HCV genotype sequences in the region targeted by the dh-siRNAs and siRNA sequences. Aligned sequences for siRNAs are indicated by shaded boxes; dots indicate matches to the consensus (HCR6); individual bases indicate genotype-specific differences. (b–e) Inhibition efficacy by mixture of three HCR6-based dh-siRNAs against homogeneous replicon cells (b), heterogeneous replicon cells with RMT-tri sequences (genotype-1b; 3 mismatches) (c), heterogeneous replicon cells with Con1 sequences (genotype-1b; 3 mismatches) (d) and heterogeneous replicon cells with JFH1 sequences (genotype-2a; 2 mismatches) (e). All samples were assessed at 72 hr after transfection. Data are presented as mean ± s.d. (n = 5) of values normalized to those obtained with mock-transfected cells.
