Figure 3 | Scientific Reports

Figure 3

From: Circulating tumor cell identification by functionalized silver-gold nanorods with multicolor, super-enhanced SERS and photothermal resonances

Figure 3

Immunocytochemistry staining (ICC) of MCF-7 cells in a mixed culture with fibroblast BJ-1 cells.

Top row, anti-EpCAM, anti-CD44, anti-Keratin 18 and anti-IGF antibodies (1:200) staining of cells followed by (1:500 of dilution) goat anti-mouse IgG (Alexa fluor 555, 594, 488 and 647), respectively. Middle row, DAPI nuclear stainings. Bottom row, merged images of two upper rows. The white arrows indicate surface labeled MCF-7 cells, while fibroblast cells are not labeled.

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