Figure 3
The effects of EPS on in vitro skeletal muscle tissues from days 7 to 10.
(a) %Pt of the EPS-treated tissue constructs on day 7. The tissue constructs were cultured with 1 Hz EPS at 0.3 V/mm and 4 ms from days 4 to 7 and %Pt was evaluated on day 7. Circles: 2 ms; triangles: 4 ms; squares: 10 ms. Data are shown as mean ± SD of three constructs. (b) EPS protocol during myogenic differentiation culture. (c) EPS culture was started at 24.5%Pt on day 4, changed to ~20%Pt (20.8 ± 3.4%Pt or 21.2 ± 5.0%Pt), ~50%Pt (54.1 ± 6.7%Pt or 54.1 ± 7.6%Pt), or ~80%Pt (79.4 ± 3.3%Pt or 79.5 ± 4.2%Pt) on day 7 and then force production was measured on day 10. Data are shown as mean ± SD of three constructs. Columns that were statistically indistinguishable (P > 0.05) are marked with the same letter.
