Figure 2
From: de novo Synthesis of a Bacterial Toxin/Antitoxin System
ArT-producing cells show “ghost”-like phenotype.
(a) Appearance of cells producing GhoS, ArT and GhoT under phase contrast at 1,000× magnification (oil immersion). GhoS: BW25113/pCA24N-ghoS, ArT: BW25113/pCA24N-arT and GhoT: BW25113/pCA24N-ghoT. (b) Localization of GFP-tagged GhoS, ArT and GhoT using epifluorescence microscopy. Note the “ghost”-like phenotype in cells producing ArT and the true “ghost” cells in GhoT-expressing cells. GhoS: BW25113/pCA24N-ghoS(GFP+), ArT: BW25113/pCA24N-arT(GFP+) and GhoT: BW25113/pCA24N-ghoT(GFP+). Scale bars denote 5 µm.
