Table 1 PCR primers used for amplification of PAX6 gene

From: A novel de novo duplication mutation of PAX6 in a Chinese family with aniridia and other ocular abnormalities

Exon

Primer Name

M13 forward primer or reverse primer + specific sequence 5′-3′

Product size(bp)

1 ~ 2

PAX6-1MF

TGTAAAACGACGGCCAGTCTCATTTCCCGCTCTGGTTC

472

 

PAX6-2MR

CAGGAAACAGCTATGACCAAGCGAGAAGAAAGAAGCGG

 

3 ~ 4

PAX6-3MF

TGTAAAACGACGGCCAGTTCAGAGAGCCCATGGACGTAT

625

 

PAX6-4MR

CAGGAAACAGCTATGACCGAAGTCCCAGAAAGACCAGA

 

5 ~ 5a

PAX6-5MF

TGTAAAACGACGGCCAGTCTCTTCTTCCTCTTCACTCTG

1117

 

PAX6-5aMR

CAGGAAACAGCTATGACCGGGAAGTGGACAGAAAACC

 

6 ~ 7

PAX6-6MF

TGTAAAACGACGGCCAGTGGTTTTCTGTCCACTTCCC

1209

 

PAX6-7MR

CAGGAAACAGCTATGACCAGCATGGAAGCCCTGAGAGGA

 

8 ~ 9

PAX6-8MF

TGTAAAACGACGGCCAGTGGGAATGTTTTGGTGAGGCT

960

 

PAX6-9MR

CAGGAAACAGCTATGACCGTACTCTGTACAAGCACCTC

 

10 ~ 11

PAX6-10MF

TGTAAAACGACGGCCAGTGTAGACACAGTGCTAACCTG

431

 

PAX6-11MR

CAGGAAACAGCTATGACCTTATGCAGGCCACCACCAGC

 

12 ~ 13

PAX6-12MF

TGTAAAACGACGGCCAGTTAATGATCAGACTTGTTGGCAG

1152

 

PAX6-13MR

CAGGAAACAGCTATGACCGAACTGAAGCGGCTCTAACA

 
  1. DNA sequencing primers: M13 forward primer (TGTAAAACGACGGCCAGT) and M13 reverse primer (CAGGAAACAGCTATGACC;
  2. PCR conditions: 94°C/5′; 94°C/30″,58°C/45″,72°C/1 ~ 2′,10 cycles; 94°C/30″,61°C/45″,72°C/1 ~ 2′,25 cycles; 72°C/5′; 4°C/∞.
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