Figure 5
From: Imaging of influenza virus sialidase activity in living cells
Histochemical visualization of genetically NA-expressed cells by using the BTP3-Neu5Ac assay.
(a) COS7 cells were transfected with the NA gene of A/duck/Hong Kong/313/4/1978 (H5N3). After culture for 24 hr, the transfected cells were incubated with 10 μM BTP3-Neu5Ac in the absence or presence of 1 μM zanamivir at 37°C for 5 min. (b) COS7 cells were transfected with each NA gene of A/Brevig Mission/1/1918 (H1N1), A/chicken/Shimane/1/2010 (H5N1) and A/Anhui/1/2013 (H7N9). After culture for 24 hr, the transfected cells were incubated with 10 μM BTP3-Neu5Ac at 37°C for 5 min. Scale bars indicate 200 μm.
