Figure 7 | Scientific Reports

Figure 7

From: Transcriptional Repressor Domain of MBD1 is Intrinsically Disordered and Interacts with its Binding Partners in a Selective Manner

Figure 7

Isothermal Titration Calorimetry of MBD1-c and its mutants with MBP-MCAF1Δ8, MBP-HDAC3, MPG and MBP.

(A) MBD1-c titrated against MBP-MCAF1Δ8 until saturation, show single binding site with molar ratio of 1. (B) MBD1-c titrated against MBP-HDAC3 until saturation, show single binding site with molar ratio of 1. (C) MBD1-c titrated against MPG until saturation, show single binding site with molar ratio of 1. (D–F) MBD1-c mutants titrated against MBP-MCAF1Δ8, MBP-HDAC3 and MPG, respectively, did not show any significant binding. (G) MBD1-c titrated against MBP did not show any significant binding.

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