Figure 5 | Scientific Reports

Figure 5

From: Nitro-oleic acid downregulates lipoprotein-associated phospholipase A2 expression via the p42/p44 MAPK and NFκB pathways

Figure 5

OA-NO2 downregulates Lp-PLA2 expression via the p65 NFκB pathway.

THP-1 cells were incubated with OA-NO2, PDTC and LPS at the indicated concentrations for 1 h and then stimulated with PMA (0.05 μM). (A), The relative Lp-PLA2 mRNA expression levels were determined after stimulation with PMA for 12 h. (B), The phosphorylation of p65 NFκB and the Lp-PLA2 protein expression levels were determined after stimulation with PMA for 24 h. (C), the NFκB/DNA binding activity was measured by EMSA.

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