Figure 2
From: Beyond Warburg effect – dual metabolic nature of cancer cells
Effect of lactic acidosis on glucose consumption and lactate generation by 4T1 cells.
(a) A titration of exogenous lactic acid on glucose consumption, net lactate production and cell division. 4T1 cells were incubated in RPMI-1640 medium supplemented with lactic acid to desired lactate concentrations as indicated, incubated for 24 hours, followed by cell counting and glucose consumption and net lactate generation determinations. L/G ratio: lactate generated over glucose consumption; C/G ratio, cells produced by consumption of 1 μ mole glucose. (b) Lactic acidosis generated by 4T1 cancer cells transit Warburg effect to a nonglycolytic phenotype. 4T1 cells were incubated in the RPMI-1640 medium containing 18 mM glucose; at indicated hours, medium glucose and lactate levels, medium pH and L/G ratios were monitored. A given L/G ratio denotes μmoles lactate generated from consuming one μmole glucose within an incubation period, e.g., the L/G ratio at 64 hours means the lactate generated between 48 and 64 hours divided by the glucose consumed in the same period. Note that the L/G ratio is correlated with the medium pH and inversely correlated with the lactate concentration. Data are mean ± SD, n = 3 and were confirmed by 2 independent experiments. * p < 0.05, ** p < 0.01, compared with control.
