Figure 2 | Scientific Reports

Figure 2

From: Direct induction of ramified microglia-like cells from human monocytes: Dynamic microglial dysfunction in Nasu-Hakola disease

Figure 2

The iMG cells show the character of human resident microglia.

(A and B) The expression levels of surface markers on the iMG cells and induced macrophage were performed by flow cytometer. Peripheral monocytes were incubated with GM-CSF (macrophage) or cocktail of GM-CSF and IL-34 (iMG cells) for 14 days. The iMG cells showed the specific phenotypes of microglia compared to macrophage. (C to E) The expression pattern of CCR2 and CX3CR1 between monocytes and iMG cells were observed by immunocytochemistry. The monocytes and iMG cells were cultured for 14 days and stained with specific antibodies. (C and D) The iMG cells were stained with bright green fluorescence (CX3CR1) bearing highly branched forms. Scale bar, 50 μm. (E) The expression ratio (CX3CR1/CCR2) of iMG cells was significantly higher than that of monocytes by flow cytometry (n = 3). The iMG cells were incubated with IL-4 (F) or dexamethasone (G) for 72 hours and extracted RNA was analyzed by qRT-PCR (n = 6). Fold changes were depicted in mRNA levels after stimulation compared with unstimulated cells. *P < 0.05. Error bars, standard error of the mean (SEM).

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