Figure 1
From: Labeling of neuronal differentiation and neuron cells with biocompatible fluorescent nanodiamonds
FNDs do not affect cell morphology, viability and apoptosis in the ECS cells.
(a) Two pluripotent embryonic carcinoma stem (ECS) cell lines, P19 and NT2/D1, were used in this study. (b) P19 and NT2/D1 cells were plated at a density of 7 × 105 cells or 4 × 105 cells per 60-mm Petri dish, respectively. Then the cells were treated with or without FND (0.1–50 μg/ml for 24 h). Representative phase-contrast photomicrographs (200× magnification) show the cell morphology of P19 and NT2/D1 cells. (c) P19 cells were plated at a density of 7 × 105 cells per 60-mm Petri dish. (d) NT2/D1 cells were plated at a density of 4 × 105 cells per 60-mm Petri dish. Then the cells were treated with or without FNDs (0.1–50 μg/ml for 24 h). At the end of treatment, the levels of apoptotic cells were determined by Annexin V-FITC staining and analyzed by flow cytometer. The Annexin V+ cells represented cells undergoing apoptosis. The populations of healthy and apoptotic cells were quantified from a minimum of 10,000 cells using CellQuest software. Results were obtained from three separate experiments and the bar represented the mean ± S.E. The percentages of healthy and apoptotic cell populations were not significantly altered by treatment FNDs in both P19 and NT2/D1 cells (p > 0.05).
