Figure 3
From: Labeling of neuronal differentiation and neuron cells with biocompatible fluorescent nanodiamonds
The uptake ability of FNDs in the ECS cells by flow cytometry.
(a) P19 cells were plated at a density of 7 × 105 cells per 60-mm Petri dish. (b) NT2/D1 cells were plated at a density of 4 × 105 cells per 60-mm Petri dish. Then the cells were treated with or without FNDs (0.1–50 μg/ml for 24 h). At the end of treatment, the cells were trypsinized and then subjected to flow cytometer. The fluorescence intensity of FNDs was excited with wavelength 488 nm and the emission was collected with > 650 nm signal range (FL3-H). The fluorescence intensity of FNDs was quantified from a minimum of 10,000 cells by CellQuest software. Results were obtained from three separate experiments and the bar represented the mean ± S.E. *p < 0.05, **p < 0.01 and ***p < 0.001 indicate significant difference between control and FND treatment.
