Figure 6
From: Labeling of neuronal differentiation and neuron cells with biocompatible fluorescent nanodiamonds
FNDs do not alter the gene and protein expressions of β-III-tubulin during neuronal differentiation.
(a) P19 ECS cells were treated with or without FNDs (50 μg/ml for 24 h) and then the cells were differentiated into neuron cells by RA induction. Total cellular RNA was purified for reverse transcription-polymerase chain reaction (RT-PCR) analysis. The RT-PCR amplified gene products of β-III-tubulin and actin were 749 and 556 bps, respectively. Actin is an internal control gene. (b) The mRNA levels were analyzed by semi-quantification. Results were obtained from three separate experiments and the bar represented the mean ± S.E. *p < 0.05 indicates significant difference between the ECS cells and differentiated cells following treatment with or without FND. (c) P19 cells were treated with or without 50 μg/ml FNDs for 24 h. P19 or differentiated neuron cells were incubated with anti-β-III-tubulin antibody and then incubated with goat anti-rabbit IgG HiLyte Fluor 488 antibody. The fluorescence intensity of β-III-tubulin in P19 cells and differentiated neuron cells were analyzed by flow cytometer. (d) The fluorescence intensity was quantified from a minimum of 10,000 cells by CellQuest software. Results were obtained from four separate experiments and the bar represents the mean ± S.E. *p < 0.05 indicates significant difference between the ECS cells and differentiated cells following treatment with or without FND.
