Figure 7
From: Labeling of neuronal differentiation and neuron cells with biocompatible fluorescent nanodiamonds
FNDs do not induce cytotoxicity and apoptosis in the neuronal differentiation of ECS cells.
(a) P19 cells were treated with FNDs (50 μg/ml for 24 h) and then differentiated into neuron cells. The differentiated cells were plated at a density of 7 × 104 cells per well in 24-well plate and the cell viability was measured by MTT assays. Results were obtained from three separate experiments and the bar represented the mean ± S.E. (b) P19 ECS cells were treated with FNDs (50 μg/ml for 24 h) and then differentiated into neuron cells. The differentiated cells were collected for flow cytometer analysis. The effect of apoptosis was determined by Annexin V-FITC staining using flow cytometer analysis. The populations of Annexin V+ cells represented cells undergoing apoptosis. (c) The populations of healthy and apoptotic cells were quantified from a minimum of 10,000 cells using CellQuest software. Results were obtained from three separate experiments and the bar represented the mean ± S.E. The percentages of healthy and apoptotic cell populations were not significantly altered by treatment with FNDs (p > 0.05).
