Figure 9

FNDs do not induce cytotoxicity and apoptosis in the differentiated neuron cells.

(a) P19 cells were differentiated into neuron cells. The neuron cells were plated at a density of 7 × 10⁴ cells per well in 24-well plate for 16–20 h. Then the cells were treated with or without FNDs (50 μg/ml for 24 h). At the end of treatment, the cell viability was measured by MTT assays. Results were obtained from three separate experiments and the bar represented the mean ± S.E. (b) P19 cells were differentiated into neuron cells by RA induction. Then the neuron cells were treated with or without FNDs (50 μg/ml for 24 h). The effect of apoptosis was determined by Annexin V-FITC staining using flow cytometer analysis. The populations of Annexin V⁺ cells represented cells undergoing apoptosis. (c) The populations of healthy and apoptotic cells were quantified from a minimum of 10,000 cells using CellQuest software. Results were obtained from three separate experiments and the bar represented the mean ± S.E. The percentages of healthy and apoptotic cell populations were not significantly altered by treatment with FNDs (p > 0.05).