Figure 1
Phylogenetic relationships among Lep1-like elements in non-lepidopteran species and representative lepidopteran insect species.
The Neighbor-joining tree was generated in MEGA5 with 1000 bootstrapping. Bootstrap values below 50% are not shown. Lep1-like elements in non-lepidopteran species were derived from database homology searches and the abbreviations and GenBank entries were described in Table 1. Consensus sequences of HaLep1 lineage A (HaLep1A CS), HaLep1 lineage B (HaLep1B CS), HaLep1 lineage C (HaLep1C CS) and AglaLep1 (AglaLep1 CS) were derived from multiple sequences alignments in this study. Trichoplusia niTnLep1_1 was obtained from database homology searches using CfLep1_1 as query and it's GenBank entry is FF372817. Other representative lepidopteran Lep1 elements were derived from Coates et al.18 and are obtained from the following GenBank entries: D86623.1 for Bombyx mori BmLep1_335, DQ242656.1 for B. moriBmLep1_87, CR974474 for Heliconius melpomene HmLep1_1, AC239123 for Bicyclus anynana BaLep1_1, FP340414 for Spodoptera frugiperda SfLep1_1, EU532470 for Ostrinia nubilalis OnLep1_1, FM995623 for Papilio dardanus PdLep1_1. Taxa showing Lep1 are colored taxonomically, with lepidopteran insects in purple, Hymenoptera wasps in green, Hemiptera aphids in light blue, Coleoptera beetle in gray, bracoviruses in red and Nosema bombycis in orange.
