Figure 2
CRP binding to sensor chip blocked with BSA or PEG-SH.
A plot comparison of the SPRi kinetic signal after the injection of CRP (2 μg/ml) in buffer (10 mM TRIS, 15 mM NaCl, 2 mM CaCl2 pH 7.4) onto a gold-coated prism that has been functionalized with biotinylated CRP-specific and control aptamer followed by blocking with (a) BSA and (b) PEG-SH. (c) A comparison of the equilibrium dissociation constant (KD) between BSA and PEG-SH treated surface to the binding response of CRP.
