Table 1 Plant genera identified in 30 LDW samples using HTS based on ITS2. Notice that P. cocos was not discovered in any LDW samples based on HTS results with universal ITS2 primers, but its existence was confirmed by specific primers on selected samples

From: Biological ingredient analysis of traditional Chinese medicine preparation based on high-throughput sequencing: the story for Liuwei Dihuang Wan

 

MH

MS

MT

RE

Sample ID

A1

A2

A3

B1

B2

B3

C1

C2

C3

A1

A2

A3

B1

B2

B3

C1

C2

C3

A1

A2

A3

B1

B2

B3

C1

C2

C3

1

2

3

Alisma orientalis

✓

 

✓

   

✓

  

✓

 

✓

✓

✓

 

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

Cornus officinalis

         

✓

✓

✓

✓

✓

✓

✓

✓

✓

    

✓

       

Dioscorea opposita

✓

 

✓

✓

✓

 

✓

✓

 

✓

✓

✓

 

✓

         

✓

 

✓

    

Paeonia suffruticosa

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

✓

Rehmannia glutinosa

✓

✓

✓

 

✓

 

✓

✓

✓

  

✓

✓

✓

✓

✓

 

✓

     

✓

      

Fabaceae

                              

- Albizia

          

✓

                   

- Glycine

        

✓

✓

✓

    

✓

              

- Robinia

✓

      

✓

                      

- Senna

        

✓

                     

- Vigna

       

✓

  

✓

✓

✓

✓

✓

✓

✓

✓

         

✓

✓

✓

Amaranthaceae

                              

- Amaranthus

         

✓

                    

Solanaceae

                              

- Capsicum

        

✓

                     

Rutaceae

                              

- Citrus

   

✓

                          

Cucurbitaceae

                              

- Cucurbita

       

✓

✓

                     

Convolvulaceae

                              

- Cuscuta

         

✓

✓

                   

Brassicaceae

                              

- Descurainia

 

✓

                            

Malvaceae

                              

- Gossypium

      

✓

✓

✓

                     

Convolvulaceae

                              

- Ipomoea

✓

✓

 

✓

✓

✓

✓

✓

✓

✓

 

✓

✓

✓

✓

               

Nyctaginaceae

                              

- Mirabilis

                  

✓

           

Paeoniaceae

                              

- Paeonia

   

✓

                          

Plantaginaceae

                              

- Plantago

           

✓

✓

✓

 

✓

✓

✓

            

Salicaceae

                              

- Populus

        

✓