Figure 5
From: Periodontitis-activated monocytes/macrophages cause aortic inflammation
Effect of periodontitis-activated MNCs on the nuclear translocation of p65 NF-κB and the expression of VCAM-1 in HUVECs.
(a) The adhesion of MNCs isolated from peripheral blood of the control and the periodontitis rats to HUVECs. MNCs were labeled with PKH26 and placed on a HUVECs monolayer for 24 h. (b) Quantification of attached PKH26-labeled MNCs (n = 5). Scale bar, 50 μm. *P < 0.05 in two-sided Student's t-test. (c) Representative images of the nuclear translocation of p65 NF-κB and the expression of VCAM-1 in HUVECs. The nuclear translocation of p65 NF-κB was detected 1 h after MNC adhesion and the expression of VCAM-1 was detected 24 h after MNC adhesion, respectively. Endothelial cell nuclei, VCAM-1 and p65 NF-κB are in blue and green, respectively. Scale bar, 20 μm. (d, e) Quantification of HUVECs positive for nuclear translocation of p65 NF-κB and the expression of VCAM-1. n = 6 to 8 visual fields. *P < 0.01 compared to MNCs from the control rats (by one-way ANOVA). All data represent the means ± s.e.m.
