Figure 3
Histopathological analysis of lung tissues after LPS injection.
Lungs were obtained from GAPDH or PBS pre-treated mice 12 h after LPS administration and embedded in paraffin. Embedded lungs were sectioned and the sections were stained with hematoxylin and eosin (a–c), Giemsa (d–f) or anti-Ly-6G antibody (h–j). The images of stained lung sections were taken using a light microscope (Olympus Corporation, ×100). Each panel represents naïve (a, d, h), PBS (b, e, i) and GAPDH pre-injected groups (c, f, j), respectively. The data are representative examples of three individual lung sections. Scale bars in all pictures show 100 μm. Giemsa-stained cell number in each image was counted (g). White, black and gray bars show naïve, PBS and GAPDH pre-treated groups, respectively. Values are presented as the mean ± S.D.
