Figure 4 | Scientific Reports

Figure 4

From: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) prevents lipopolysaccharide (LPS)-induced, sepsis-related severe acute lung injury in mice

Figure 4

Proinflammatory cytokine expression in the lung after LPS injection.

Lungs were obtained from GAPDH or PBS pre-treated mice at 6 or 12 h after LPS administration and pulmonary mRNA and protein were extracted. Gene expression of cytokines (IL-6 (a), TNF-α (b) and IFN-γ (c)) in the lung was determined by real-time PCR. White, black and gray bars show naïve, PBS and GAPDH pre-treated groups, respectively. Values are presented as the mean ± S.D. Cytokine level (IL-6 (d), TNF-α (e) and IFN-γ (f)) in the lung was measured by using specific ELISA kit. Each symbol in all graphs indicates individual mouse and bars show mean value. NS: not significant. *, P < 0.05 versus the PBS pre-treated group.

Back to article page