Figure 2 | Scientific Reports

Figure 2

From: A novel proteasome inhibitor suppresses tumor growth via targeting both 19S proteasome deubiquitinases and 20S proteolytic peptidases

Figure 2

Pyrithione (PT) and H2O2 in combination enhanced cytotoxicity.

(a) PT and H2O2 synergistically decreased cell viability. U266 cancer cells were treated with PT, H2O2 alone and their combination at the indicated doses for 24 hours, cell viability was detected by MTS assay. Mean ± SD (n = 3). *P < 0.05, versus each PT treatment alone. (b and c) PT and H2O2 in combination accelerated cell apoptosis and cell death in U266 cells. U266 cells were exposed to PT, H2O2 and their combination at the indicated doses for 24 hours, cell death and cell apoptosis were detected by either PI staining with an inverted fluorescence microscope in live cells (b) or by Annexin V/propidium (PI) staining with flow cytometer (c). Scale bar = 50 μm.

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