Figure 8 | Scientific Reports

Figure 8

From: A novel proteasome inhibitor suppresses tumor growth via targeting both 19S proteasome deubiquitinases and 20S proteolytic peptidases

Figure 8

CuPT inhibits tumor growth and the ubiquitin-proteasome system in vivo.

(a–f) Nude mice bearing HepG2 xenograft tumors were treated with vehicle and CuPT (2.5 mg/kg/d) for totally 15 days (Day 7 interval) after inoculation of HepG2 cells. On day 18 after inoculation, the mice were sacrificed and the tumor tissues were weighed, imaged and summarized. Body weight was recorded everyday. (a) Body weight changes; (b) Tumor weight and image. *P < 0.05, versus CuPT-treated group; (c) Proteasome substrate protein changes. Proteasome-related proteins in tumor tissues were detected by immunohistological analysis. All the immunostaining was repeated in three mouse tumor tissues and the most typical images were shown. (g and h) Nude mice bearing NCI-H929 xenograft tumors were treated with vehicle and CuPT (2.5 mg/kg/d), for 5 days after inoculation of NCI-H929 cells. On day 8 after inoculation, the mice were sacrificed. The tumor tissues were weighed and proteasome-related proteins in tumor tissues were detected by immunohistological analysis.

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